PRIMARY CULTURE
OF CENTRAL NERVOUS SYSTEM CELLS
(TYPE I ASTROCYTES AND NEURONS in defined medium, with astrocyte
feeder layer in defined medium, and in
serum containing media)(1991-to date)
PRIMARY CULTURE OF PITUITARY CELLS AND HUMAN DERIVED ADENOMATOUS CELLS
(1986-90)
VITALITY TESTS (TRYPAN BLUE EXCLUSION TEST, PROPIDIUM AND
FLUORESCEIN DETERMINATION OF VITALITY, MTT, EXOSAMINIDASE ACTIVITY ASSAY)
Proliferation assessment: cell count, [3]H-THYMIDINE INCORPORATION,
cell number detection by evaluation of exosaminidase levels
Morphology assessment on neurons
IMMUNOCYTOCHEMISTRY TECHNIQUES: indirect immunofluorescence on cell
lines and primary cultures of cortical neurons, type I astrocytes, hippocampal
neurons, striatal neurons.
BIOASSAY OF IL-6 ACTIVITY (7TD1 HYBRIDOMA)(1991-to date)
KNOWLEDGE OF DYNAMIC PERIFUSION TECHNIQUE (1987-1992)
BASIC KNOWLEDGE OF REVERSE HEMOLYTIC PLAQUE ASSAY (1990-1992)
CYTOSOLIC
CALCIUM CONCENTRATION MEASUREMENT (FURA-2, QUIN-2) (PERFORMED ON CELLULAR
SUSPENSIONS, AND IN SINGLE CELLS)(1987-to date)
INOSITOL
PHOSPHATES ASSAY (1987-to date)
ADENYLATE CYCLASE ASSAY:
membrane preparations and intracellular accumulation
Calcium imaging
Immunocytochemistry
Confocale microscopy
Viability of neurons with vital dye
IMMUNOBLOTTING
PROCEDURES (1989)
BASIC
KNOWLEDGE ABOUT RADIOIMMUNOASSAYS (1987-to date)
BASIC
KNOWLEDGE OF PROTEIN PHOSPHORYLATION ASSAYS
(1989)
BASIC KNOWLEDGE OF NORTHERN BLOT ANALISYS (1992-to date)
Transfection of cell lines
Clonal selection of transfected cell lines
DISSECTION OF ALL
THE MOST USED RAT CEREBRAL AREAS (FRONTAL CORTEX,
TEMPORAL CORTEX, PARIETAL CORTEX,
HIPPOCAMPUS, HYPOTHALAMUS, STRIATUM, CEREBELLUM) (1986-to date)
BASIC KNOWLEDGE
ABOUT THE DISSECTION
OF OTHER RAT ORGANS (KIDNEY, LIVER, SPLEEN, ADRENAL GLANDS)(1986-to date)
ANIMAL Learning techniques (PASSIVE AVOIDANCE, ACTIVE AVOIDANCE, LOCOMOTOR ACTIVITY)(1986-to date)